A new genus, Pseudochetoneura gen. nov., is proposed for a single new species, P. otongensis sp. nov. (Diptera: Keroplatidae), collected in the Otonga Reserve in Ecuador. Its possible relationships with other genera of Keroplatidae are briefly discussed. The recently described genus Bisubcosta Papp, 2006 is considered a junior synonym of Chetoneura Colless, 1962, which now includes three species, Ch. cavernae Colless, 1962, Ch. oligoradiata (Papp, 2006) comb. nov., and Ch. shennonggongensis Amorim & Niu, 2008.