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Two species of Hydrellia Robineau-Desvoidy, 1830 (Diptera: Ephydridae) are newly described: Hydrellia dolezali sp. nov. and H. pilsna sp. nov., both from the Czech Republic. Redescriptions and new records of H. aurifer Cresson, 1932 and H. frontalis Loew, 1860 from the Czech Republic and the United Kingdom are given. The first case of sexual dimorphism in wing coloration in Hydrellia is provided.
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