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The present study discusses the importance of citizen science as a form of scientific research, defined by the participation of the general public. An important factor influencing the success of these projects, although still little studied, is the participants‘ motivation. Based on the results of a questionnaire survey (n = 94), the study tries to outline the issues of motivation and involvement of the general public on the example of citizen science project City Nature Challenge: Prague, which uses the mobile application iNaturalist. The results of the study show that the project participants see its importance in mapping wildlife in collaboration with the public, and at the same time in popularizing urban biodiversity and the need for its protection. It is obvious that a stable community of volunteers interested in nature are emerging around the City Nature Challenge project. We need this community to be properly motivated and further studied in terms of positive motivation, changes in behavior and attitudes.
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