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Chalarus Walker, 1834 (Diptera: Pipunculidae: Chalarinae) has a cosmopolitan distribution, with 11 known species in the Neotropical Region, but none recorded in Colombia. Two new species of Chalarus are described from Colombia, namely Chalarus boyacensis sp. nov. (type locality: Cabaña Chaina, Santuario de Fauna y Flora Iguaque) and C. chairensis sp. nov. (type locality: Vereda Tigrera Alta, Cartagena del Chaira). Chalarus absonus Rafael, 1990, C. delicatus Rafael, 1990 and C. connexus Rafael, 1988 are recorded in Colombia for the first time, with amended and comparative diagnoses. Photographs of five South American species, distribution map and a dichotomic key to males of all Neotropical species are included.
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